The effect of 4-hexylresocinol administration on SCC-9 cells: mass spectrometric identification of proteins and cDNA microarray analysis
강예진, 김성곤,
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강예진 ( Kang Yei-Jin ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
김성곤 ( Kim Seong-Gon ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Background: In stress situations, bacteria produce dormancy-inducing factors to stop cell growth. The dormancy-inducing factors may have an inhibitory effect on tumor cell growth. Here we analyzed the differentially expressed protein profiles after 4-hexylresorcinol (4HR), one of the dormancy-inducing factors, administration using in vitro oral squamous carcinoma cells (SCC-9).
Method: The control group was SCC-9 cells culture without 4HR administration. The experimental group received 10?μg/mL of 4HR. Collected proteins from each group were loaded for 2D electrophoresis. Among the separated proteins, 20 differentially expressed proteins were selected and processed for LC-MS/MS.
Results: In proteomic analysis, the expression of keratin 1, keratin 10, and histone H2B were increased. In cDNA microarray assay, the genes related to the cellular differentiation (involucrin, keratin 13, 14) were highly expressed in the 4HR treated group (fold ratio >?2.0; Table 2). Interestingly, histone family was upregulated in the cDNA microarray assay.
Conclusion: The administration of 4HR on SCC-9 cells increased epithelial cell differentiation markers and histone.
키워드
4-hexylresorcinol; Oral squamous cell carcinoma; Keratin; Histone
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